University of North Texas 
- Any individual that operates the autoclaves must successfully complete a lab-specific or RMS given training on safe operating procedures for autoclaves.
- Do NOT autoclave any chemicals.
- Read the owner's manual before using the autoclave for the first time.
- Operating instructions should be posted near the autoclave.
- Follow the manufacturer's directions for loading the autoclave.
- Do not overload the autoclave compartment and allow for enough space between items for the steam to circulate.
- Be sure to close and latch the autoclave door.
- Be aware that liquids, especially in large quantities, can be superheated when the autoclave is opened. Jarring them may cause sudden boiling, and result in burns.
- At the end of the run, open the autoclave slowly: first open the door only a crack to let any steam escape slowly for several minutes, and then open all the way. Opening the door suddenly can scald a bare hand, arm, or face.
- Large flasks or bottles of liquid removed immediately from the autoclave can cause serious burns by scalding if they break in your hands. Immediately transfer hot items with liquid to a cart; never carry in your hands.
- Wear appropriate PPE, including eye protection and insulating heat-resistant gloves.
When using glass under vacuum, some general guidelines to follow:
- Inspect glassware that will be used for reduced pressure to make sure there are no defects such as chips or cracks that may compromise its integrity.
- Only glassware that is approved for low pressure should be used. Never use a flat bottom flask (unless it is a heavy walled filter flask) or other thin-walled flask that are not appropriate to handle low pressure.
- Use a shield between the user and any glass under vacuum or wrap the glass with tape to contain any glass in the event of an implosion.